VOUCHER SPECIMENS

Three methods of storage of vouchers are used:

1. Intact spores are stored on microscope slides in a drop of glycerin or lactic acid. The slides are placed in Petri dishes.
2. Intact spores and mycorrhizal root segments are stored in vials with formaldehyde.
3. Spores are permanently mounted on microscope slides and crushed in both polyvinyl alcohol, lactic acid, glycerin (PVLG) and PVLG mixed with Melzer’s reagent (1:1 v/v). At present, over 3000 slides of crushed spores are stored. Mycorrhizal roots are preserved in PVLG and vials with ethanol. Slides are stored in numbered boxes. Each box has a reference number corresponding with a given described or undescribed species. These numbers correspond with numbers of microscopic slides with spores (see point 1), vials, and photographs.