(Nicol. & N.C. Schenck) C. Walker & F.E. Sanders
In PVLG+Melzer's reagent
In PVLG+Melzer's reagent
Spore wall composed of two layers (swl1-3).
Layer 1 permanent, smooth, hyaline, (1.0-)1.5(-2.5) µm thick, better visible in spores crushed in Melzer’s reagent, in which it is nonreactive in contrast to layer 2 staining darkly in this reagent; this layer frequently slightly separates from layer 2 in spores mounted in lactic acid-based mountants.
Layer 2 laminate, hyaline to yolk yellow (4B8), (3.0-)7.1(-9.2) µm thick, staining dark purple (14F8) in Melzer’s reagent.
Germination wall 1 contains two layers (gw1l1 and 2).
Layer 1 flexible, hyaline, <0.5 µm thick.
Layer 2 flexible, hyaline, (0.8-)1.2(-1.9) µm thick.Layer 1 flexible, coriaceous, hyaline, (1.5-)2.8(-5.3) µm thick, usually reddish white (8A2) to pale red (8A3) in Melzer’s reagent.
Layer 2 plastic, hyaline, 5-25 µm thick in PVLG, 1.0-3.0 µm thick and beetroot purple (13D8) in Melzer’s reagent.
Structure of sporogenous cell composed of two layers, continuous with spore wall layers 1 and 2.
Layer 1 hyaline, <0.3-0.5 µm thick, very difficult to see.
Layer 2 hyaline to yolk yellow (4B8), 1.8-3.0 µm thick at the spore base.
GERMINATION SHIELD ellipsoid; hyaline to pale yellow (4A3); 65-80 x 100-120 µm; with deep folds partitioning 4-7 lobes with smooth margins; formed on germination wall 2.
AUXILIARY CELLS rarely single in the soil, usually in loose clusters of 2-10; hyaline to pale yellow (4A3); pyriform to irregular; 30.0-42.0 µm; produced on coiled hyphae, 2.2-5.8 µm diam, concolorous with auxiliary cells.
MYCORRHIZAE. Spores of S. pellucida have been found associated in the field with many cultivated and uncultivated plant species (Blaszkowski 1989, 1993a, b, 1994; Blaszkowski et al. 2002; Tadych and Blaszkowski 2000). Attempts to establish one-species cultures of this fungus failed.
DISTRIBUTION. In Poland, S. pellucida has been widely distributed in both cultivated and uncultivated soils (Blaszkowski 1989, 1993a, b; Iwaniuk and Blaszkowski 2004). However, this fungus has generally occurred infrequently. Most of the S. pellucida specimens collected in Poland came from dune soils, including those of the Hel Peninsula (54o47’N, 18o25’E-54o36’N, 18o49’E; Blaszkowski 1994), the Gdansk and Szczecin coasts (Blaszkowski 1993b), Slowinski National Park (54o45’N, 17o26’E; Tadych and Blaszkowski 2000), and the Bledowska Desert (50o22’N, 19o34’E; Blaszkowski et al. 2002).
Scutellospora pellucida occurs in the whole world. This fungus has originally been described from spores isolated from a soybean-rhizosphere soil collected in Florida (Nicholson and Schenck 1979). Later, spores of S. pellucida have been found, e. g., in other states of the USA (Hetrick and Bloom 1983; Koske 1987; Miller et al. 1985; Rose 1988), Italy (Giovannetti 1985), Israel and Turkey (Blaszkowski et al. 2001; Blaszkowski, pers. observ.).
These fungi differ in the subcellular structure of spores and the biochemical properties of its components. The subcellular structure of spores of S. pellucida and S. calospora is identical, whereas spores of S. fulgida lack either the third spore wall layer and the germination wall 2 of the two former species (Morton 1995, 2000). Additionally, the laminate spore wall layer of only S. pellucida spores stains red in Melzer’s reagent.
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